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Science International Vol. 1 (3), 2013
Research Article
Molecular and Recombinational Characterization of Begomovirus Infecting an Ornamental Plant Alternanthera sessilis: A New Host of Tomato Leaf Curl Kerala Virus Reported in India
Avinash Marwal , Anurag Kumar Sahu , Rajneesh Prajapat , Devendra Kumar Choudhary and Rajarshi Kumar Gaur
 
Abstract: Begomovirus associated symptoms were observed in Alternanthera sessilis ornamentals growing in the gardens of Lakshmangarh, Rajasthan (India). Amplification of a PCR product was found up to the expected size (~550 bp). The PCR product was cloned and sequenced and it was utilized for molecular characterization. Recombination plays a key role in the evolution of begomovirus and may be contributing to the emergence of new species. With the development of computational recombination detection tools and an increasing number of available genome sequences, many studies have reported evidence of recombination. Various recombination events suggested that interspecific recombination has resulted in significant diversity among begomovirus. Sequence analysis showed closed similarity (98 %) with Tomato leaf curl Kerala virus, substantiating its new host as Alternanthera sessilis.
 
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